In a video posted online on Tuesday evening, Queen Sofia—the mother of the current King of Spain, Felipe VI—and Queen Letizia, his wife, could be seen physically disagreeing about some matter related to Leonor, Princess of Asturias, the heir to the Spanish throne.

The royal party of two kings, two queens and two princesses was preparing to leave Palma Cathedral in Mallorca following an Easter Sunday service—official photos from the Royal Household confirm they were all wearing the same clothes as in the video—when Queen Sofia appeared to position herself with her two granddaughters for a...